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Image Search Results
Journal: Frontiers in Immunology
Article Title: Sea Urchin Extracellular Proteins Design a Complex Protein Corona on Titanium Dioxide Nanoparticle Surface Influencing Immune Cell Behavior
doi: 10.3389/fimmu.2019.02261
Figure Lengend Snippet: The interaction between Sea urchin secretome and TiO 2 NPs, extracellular signaling and protein corona identification. (A) One-dimensional profiling of the formed non-cell-conditioned protein corona at 1, 10, and 100 μg mL −1 TiO 2 NPs after 24 h of incubation. (B) Two-dimensional profiling of the TiO 2 NP protein corona after 24 h of incubation in the non-cell-conditioned medium (Cell free CF plus CCM). (C) One-dimensional profiling of the protein corona formed in situ (in vitro) at 3 and 24 h. (D) Representative immunoblotting results show limited influence of the TiO 2 NPs on sea urchin extracellular signaling and reveal the main constituents shaping the cell-conditioned protein corona ( Pl -toposome, Pl -galectin-8, Pl -nectin, actin, tubulin). * , see text.
Article Snippet: Samples were processed with ReadyPrep™ 2-D Cleanup Kit and ReadyPrep Reduction Alkylation Kit (BioRad), quantified by
Techniques: Incubation, In Situ, In Vitro, Western Blot